A systematic review «Koumans EH, Johnson RE, Knapp JS, St Louis ME. Laboratory testing for Neisseria gonorrhoeae by recently introduced nonculture tests: a performance review with clinical and public health considerations»1 including 21 studies with a total of 17,737 samples was abstracted in DARE. There were 572 culture-positive female samples and 901 culture-positive male samples, and 92 other or total culture-positive samples. In detecting gonococcal infections of the endocervix the sensitivity of nucleic acid hybridisation was 92.1% and specificity was 99.1%. For LCR the sensitivity was 96.7% and the specificity 99.1%). In detecting gonococcal infection of the male urethra, the sensitivity of nucleic acid hybridisation was 96.4% and specificity was 98.8%. For LCR the sensitivity was 98.6% and the specificity was 99.97%. For detecting gonococcal infection in a urine specimen, the sensitivity of LCR for women was 96.2% and the specificity was 100.0%. For men, the sensitivity was 98.3% and the specificity was 100.0%.
A study «Chernesky M, Jang D, Gilchrist J et al. Head-to-head comparison of second-generation nucleic acid amplification tests for detection of Chlamydia trachomatis and Neisseria gonorrhoeae on urine samples »2 comparing 4 second-generation nucleic acid amplification tests performed with self-collected vaginal swab and first-void urine included 575 women. Vaginal swabs indicated more infections than did urine specimens in all assays. The prevalence rates were 9% (53/575) for Chlamydia trachomatis and 2% (11/575) for Neisseria gonorrhoeae. The clinical sensitivities for C. trachomatis for vaginal specimens varied from 90.0 to 98.1% and for urine specimens from 75.5 to 88.7%. Clinical sensitivities of the assays for N. gonorrhoeae, with limited positive results, ranged from 63.6% to 100%. Specificities for both infections ranged from 98.4 to 100%.