Takaisin

Associations between supplement intake and sleep quality among general population

Näytönastekatsaukset
Tarja Kaipainen
2.6.2026

Level of evidence: C

Vitamin D, amino acids, melatonin, probiotics, and L-theanine supplementation may increase the sleep quality compared with placebo among general population.

Although the evidence is based on randomized controlled trials and meta-analyses, the risk of bias, inconsistent results, indirectness in relation to insomnia treatment, imprecision, and potential publication bias significantly reduce the certainty. The overall evidence is limited by small sample sizes, short intervention periods, heterogeneity between studies, and a reliance on self-reported sleep measures.

Table 1. Description of the included studies
Reference Study type Population Intervention and comparison Outcomes Risk of bias [Table «Additional comments for included studies...»2 Additional comments]
RCT=randomized controlled trial; SR=systematic review; MA=meta-analysis
«Abboud M. Vitamin D Supplementation and Sleep: A S...»1 SR, MA adults
Iran, USA, New Zeeland, Turkey, China, Netherlands
interventions studies Sleep quantity, quality (PSQI) Moderate
«Chan V, Lo K. Efficacy of dietary supplements on i...»2 SR, MA Adults
Malaysia, Iran, United Kingdom, Australia, Israel, Turkey, USA; Germany, Italy, France
RCTs Different sleep quality parameters Moderate
«Santi D, Debbi V, Costantino F, et al. Microbiota ...»3 SR, MA Children, adults (probiotic only among adults)
N/A
RCTs Different sleep quality parameters Moderate
«Bulman A, D'Cunha NM, Marx W, et al. The effects o...»4 SR, MA All ages
Japan, Asia, USA, Europe, Australia
RCTs Different sleep quality parameters Low
Table 2. Additional comments for included studies
Reference Comments
«Abboud M. Vitamin D Supplementation and Sleep: A S...»1 The nature of studies, risk of bias.
«Chan V, Lo K. Efficacy of dietary supplements on i...»2 Risk of bias, partially blinding incomplete, some studies with industry funding
«Santi D, Debbi V, Costantino F, et al. Microbiota ...»3 Small sample size, risk of bias.

Results

Table 3. Sleep quality
Reference Number of studies and number of patients (I/C) Absolute number of events (%) I Absolute number of events (%) C Relative effect (95% CI)
Level of evidence: low
*Statistically significant
The quality of evidence is downgraded due to (study limitations, inconsistency, indirectness, imprecision, publication bias). The quality of evidence in upgraded due to large or very large effects, dose-response gradient, effect of plausible residual confounding).
I= intervention; C=comparison; CI=confidence interval: MD=mean difference; SMD Standard mean difference
«Abboud M. Vitamin D Supplementation and Sleep: A S...»1 Vitamin D supplementation
3 studies (111/112)
N/A N/A MD -2.33 *
(-3.09 to -1.57)
moderate
«Chan V, Lo K. Efficacy of dietary supplements on i...»2 Amino acid supplementation
2 studies (40/38)
N/A N/A MD -1.27 *
(-2.35 to -0.20
moderate
Melatonin supplementation
7 studies (205/199)
N/A N/A MD −1.21 *
(-2.17 to -0.24)
low
Vitamin D
4 studies (200/202)
N/A N/A MD −1.63 *
(-3.15 to -0.10)
low
Omega-3
2 studies (73/63)
N/A N/A MD 0.13
(-0.84 to 1.09)
very low
«Santi D, Debbi V, Costantino F, et al. Microbiota ...»3 Probiotics
8 studies (274/261)
N/A N/A MD -0.40 *
(-0.79 to -0.02)
moderate
«Bulman A, D'Cunha NM, Marx W, et al. The effects o...»4 L-theanine
12 studies (454/444)
N/A N/A SMD 0.44 *
(0.04 to 0.84)
moderate

References

  1. Abboud M. Vitamin D Supplementation and Sleep: A Systematic Review and Meta-Analysis of Intervention Studies. Nutrients 2022;14(5): «PMID: 35268051»PubMed
  2. Chan V, Lo K. Efficacy of dietary supplements on improving sleep quality: a systematic review and meta-analysis. Postgrad Med J 2022;98(1158):285-293 «PMID: 33441476»PubMed
  3. Santi D, Debbi V, Costantino F, et al. Microbiota Composition and Probiotics Supplementations on Sleep Quality-A Systematic Review and Meta-Analysis. Clocks Sleep 2023;5(4):770-792 «PMID: 38131749»PubMed
  4. Bulman A, D'Cunha NM, Marx W, et al. The effects of L-theanine consumption on sleep outcomes: A systematic review and meta-analysis. Sleep Med Rev 2025;81():102076 «PMID: 40056718»PubMed